| 质粒名称: | pF155 pcDNA3.1(+)SOD1G93A |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 26401 |
| 存储实验室: | Elizabeth Fisher |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 (+) |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SOD1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 470 bp |
| 基因突变: | G93A |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | XhoI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pF155 pcDNA3.1(+)SOD1G93A was a gift from Elizabeth Fisher (Addgene plasmid # 26401 ; http://n2t.net/addgene:26401 ; RRID:Addgene_26401) |
| 参考文献部分: | Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM. PLoS One. 2010 . 5(3):e9541. 10.1371/journal.pone.0009541 PubMed 20221404 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
SOD1G93A质粒图谱.png)
载体简介
载体序列
LOCUS pF155 pcDNA3.1(+)SOD1G93A 5924 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pF155 pcDNA3.1(+)SOD1G93A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5924) AUTHORS Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM TITLE Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). JOURNAL PLoS One. 2010 . 5(3):e9541. PUBMED 20221404 REFERENCE 2 (bases 1 to 5924) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5924 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 28..407 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 408..611 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 562..582 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 656..675 /label=T7 /note="T7 promoter, forward primer" promoter 656..674 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1256..1273) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1262..1486 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1532..1960 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1619..1638) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1829..1850 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(1969..1989) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1974..2303 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2154..2289 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2216..2235 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2370..3164 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2424..2443) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3034..3053 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3338..3459 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3375..3394) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3429..3448 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3508..3524) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3508..3524) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3521..3543) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3532..3548 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3556..3586) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3601..3622 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3791..3808) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3962..4550) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4042..4061) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4721..5581) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5344..5363 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5582..5686) /gene="bla" /label=AmpR promoter primer_bind complement(5761..5780) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata 661 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct 721 cggatccact agtccagtgt ggtggaattc catggcgacg aaggccgtgt gcgtgctgaa 781 gggcgacggc ccagtgcagg gcatcatcaa tttcgagcag aaggaaagta atggaccagt 841 gaaggtgtgg ggaagcatta aaggactgac tgaaggcctg catggattcc atgttcatga 901 gtttggagat aatacagcag gctgtaccag tgcaggtcct cactttaatc ctctatccag 961 aaaacacggt gggccaaagg atgaagagag gcatgttgga gacttgggca atgtgactgc 1021 tgacaaagat gctgtggccg atgtgtctat tgaagattct gtgatctcac tctcaggaga 1081 ccattgcatc attggccgca cactggtggt ccatgaaaaa gcagatgact tgggcaaagg 1141 tggaaatgaa gaaagtacaa agacaggaaa cgctggaagt cgtttggctt gtggtgtaat 1201 tgggatcgcc caataacgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg 1261 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 1321 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 1381 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 1441 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 1501 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 1561 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 1621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 1681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 1741 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 1801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 1861 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 1921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 1981 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 2041 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 2101 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 2161 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 2221 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 2281 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 2341 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 2401 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 2461 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 2521 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 2581 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 2641 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 2701 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 2761 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 2821 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 2881 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 2941 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 3001 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 3061 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 3121 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 3181 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 3241 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 3301 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa 3361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 3421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac 3481 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 3541 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 3601 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3661 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3721 tgggcgctca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 3781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 3841 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 3901 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 3961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 4141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 4201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 4261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 4321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 4381 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 4441 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 4501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 4561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 4621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 4681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 4861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 5041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 5101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 5161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 5221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 5281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 5341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 5401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 5461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 5521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5701 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag 5761 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga 5821 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa 5881 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttc //
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